Haitian variant tcpA in Vibrio cholerae O1 El Tor strains in Kolkata, India.
نویسندگان
چکیده
Priyanka Ghosh, Arindam Naha, Surajit Basak, Santanu Ghosh, T. Ramamurthy, Hemanta Koley, Ranjan K. Nandy, Sumio Shinoda, Haruo Watanabe, Asish K. Mukhopadhyay Division of Bacteriology, National Institute of Cholera and Enteric Diseases (NICED), Kolkata, India; Department of Molecular Biology & Bioinformatics and Bioinformatics Centre, Tripura University, Tripura, India; Collaborative Research Center of Okayama University for Infectious Diseases at NICED, Kolkata, India; National Institute of Infectious Diseases, Tokyo, Japan
منابع مشابه
Genetic Traits of Vibrio cholerae O1 Haitian Isolates That Are Absent in Contemporary Strains from Kolkata, India
The world's worst cholera epidemic in Haiti (2010) coerced to trace the origin and dissemination of the causative agent Vibrio cholerae O1 for proper management of cholera. Sequence analysis of the Haitian strain showed several variations in the genes encoding cholera toxin B subunit (ctxB); toxin-co-regulated pilus (tcpA), repeat in toxins (rtxA), quinolone resistance-determining region (QRDR)...
متن کاملHybrid & El Tor variant biotypes of Vibrio cholerae O1 in Thailand
BACKGROUND & OBJECTIVES El Tor Vibrio cholerae O1 carrying ctxB C trait, so-called El Tor variant that causes more severe symptoms than the prototype El Tor strain, first detected in Bangladesh was later shown to have emerged in India in 1992. Subsequently, similar V. cholerae strains were isolated in other countries in Asia and Africa. Thus, it was of interest to investigate the characteristic...
متن کاملDevelopment and evaluation of a PCR assay for tracking the emergence and dissemination of Haitian variant ctxB in Vibrio cholerae O1 strains isolated from Kolkata, India.
A PCR-based assay was developed to discriminate the classical, El Tor, and Haitian types of ctxB alleles. Our retrospective study using this newly developed PCR showed that Haitian ctxB first appeared in Kolkata during April 2006, and 93.3% of strains isolated during 2011 carried the new allele. Dendrogram analysis showed a pulsed-field gel electrophoresis (PFGE) pattern of the new variant stra...
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The emergence and spread of Vibrio cholerae O1 El Tor variant strains causing severe diarrhea has been witnessed worldwide in recent years. In the state of Odisha, India, the spread of the V. cholerae O1 El Tor variant strains was studied during outbreaks in 2008 and 2009. Analysis of 194 V. cholerae O1 Ogawa strains revealed that V. cholerae O1 El Tor variant strains are spreading gradually th...
متن کاملMolecular characterization of a new variant of toxin-coregulated pilus protein (TcpA) in a toxigenic non-O1/Non-O139 strain of Vibrio cholerae.
A toxigenic non-O1/non-O139 strain of Vibrio cholerae (10259) was found to contain a new variant of the toxin-coregulated pilus (TCP) protein gene (tcpA) as determined by PCR and Southern hybridization experiments. Nucleotide sequence analysis data of the new tcpA gene in strain 10259 (O53) showed it to be about 74 and 72% identical to those of O1 classical and El Tor biotype strains, respectiv...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 52 3 شماره
صفحات -
تاریخ انتشار 2014